Trichosanthes kirilowii Ethanol Extract and Cucurbitacin D Inhibit Cell Growth and Induce Apoptosis through Inhibition of STAT3 Activity in Breast Cancer Cells
Figure 2
TKE and CuD cause apoptosis and G2/M cell cycle arrest in MDA-MB-231 cells. (a) MDA-MB-231 cells were treated with 60 μg/mL of TKE or 1 μg/mL of CuD for 24 h. Cell cycle distribution was analyzed using a FACStar flow cytometer. (b) Graphs shows FACS-based subG1 phase distribution of MDA-MB-231 cells incubated with 60 μg/mL TKE for 24 h. (c) Bar graphs show FACS-based cell cycle distribution of MDA-MB-231 cells incubated with 60 μg/mL TKE for 24 h. (d) Bar graphs show FACS-based cell cycle distribution of MCF7 cells and MDA-MB-231 cells incubated with 60 μg/mL TKE or 1 μg/mL of CuD for 24 h. The error bars represent the mean ± standard deviation (SD).
