Trichosanthes kirilowii Ethanol Extract and Cucurbitacin D Inhibit Cell Growth and Induce Apoptosis through Inhibition of STAT3 Activity in Breast Cancer Cells
Figure 4
TKE and CuD suppress STAT3 phosphorylation and inhibit the translocation of STAT3 to the nucleus. (a) Cells were treated with 60 μg/mL TKE or 1 μg/mL CuD for different time periods (0–24 h). Total cell lysates were subjected to western blot analysis using specific antibodies (upper part) and the relative level of pSTAT3 as measured from the western blot analysis by densitometry (lower part). (b) MDA-MB-231 cells were plated in 8-well chamber slide. The next day, cells were incubated with 60 μg/mL TKE or 1 μg/mL CuD for 12 h and submitted to immunocytofluorescence assay. STAT3 distribution was exhibited by green fluorescence, and nuclei were counter-stained by DAPI.
