ERK1/2-Egr-1 Signaling Pathway-Mediated Protective Effects of Electroacupuncture in a Mouse Model of Myocardial Ischemia-Reperfusion
Figure 1
Comparison of the myocardial Egr-1and p-ERK1/2 expression at different time points after I/R. The protein levels of Egr-1and p-ERK1/2 at varying reperfusion time points (R0, R3, R6, and R24) were determined by western blot (a) and the corresponding densitometric analysis is shown in (b, c). Besides, the mRNA levels of Egr-1 were measured by qRT-PCR with data presented as relative fold increase versus sham control (d). For Egr-1 and p-ERK1/2, nearly all time points exhibit significant increase comparing with sham controls and peak levels are observed at R3 time point (, R3 versus SHAM). Six mice were sacrificed at each time points for protein and mRNA determination. R0 = ischemia for 1 h; R3 = ischemia for 1 h + reperfusion for 3 h; R6 = ischemia for 1 h + reperfusion for 6 h; R24 = ischemia for 1 h + reperfusion for 24 h. Data are presented as mean ± SEM; and versus sham control.
