Effect of Repeated Electroacupuncture Intervention on Hippocampal ERK and p38MAPK Signaling in Neuropathic Pain Rats

Figure 4

Effect of EA on expression levels of hippocampal ERK1/2mRNA and ERK1/2 and p- ERK1/2 protein in different groups. Hippocampal ERK1/2mRNA expression levels were assessed by real-time PCR and ERK1/2 protein expressions were detected by Western blot. Data are presented as mean ± SD (, compared with the sham control group; , compared with the CCI group; in each group for real-time PCR; for each group for western blot); (a) histograms show the expression levels of ERK1/2 mRNA. (b) The top panel shows the representative immunoblots of ERK1/2 proteins in the 5 groups: (1) sham control group, (2) CCI group, (3) CCI + EA2D group, (4) CCI + EA1W group, and (5) CCI + EA2W group. The histograms show relative expression levels of ERK1/2 proteins in the 5 groups. (c) The upper panel shows the representative immunoblots of p-ERK1/2 proteins in the 5 groups. The lower bar graph shows the relative expression of p-ERK1/2 proteins in the 5 groups.

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