Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species

Figure 2

Gambogic acid lysinate can inhibit MCF-7 cell proliferation; however NAC can block it. (a) Exponential growth MCF-7 cells were treated with various concentrations of gambogic acid lysinate (0, 0.25, 0.5, 1, 2, 4, and 8μmol/L) for 48 h; (b) exponential growth MCF-7 cells were treated with 2μmol/L gambogic acid lysinate for different times (0, 6, 12, 24, 36, 48, and 60 h); (c) exponential growth MCF-7 cells were treated with various concentrations of gambogic acid lysinate (0, 0.25, 0.5, 1, 2, 4, and 8μmol/L) plus various concentrations of NAC (0, 1, 2, and 4 mmol/L) for 48 h, and cell viability was detected by MTT method.

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