Review Article
CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
Table 2
Delivery of CRISPR/Cas9 components in different formats.
| ā | Plasmid DNA | Cas9 mRNA + sgRNA | Cas9 Protein + sgRNA |
| Efficiency | ++++ | ++ | ++ | Specificity | ++ | ++++ | ++++ | Cell mortality | ++++ | + | + | Off-targets | ++++ | + | + | Stress to cells | ++++ | + | + | Onset of action | + | ++ | ++++ | Half life | ++++ | ++ | + | Risks of random integration | ++++ | # | # |
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#, no risk of integration.
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