MHC Class I Presented T Cell Epitopes as Potential Antigens for Therapeutic Vaccine against HBV Chronic Infection
Figure 4
HBV specific peptides stimulate CD8+ T cell activation in vitro. (a) HLA-A2 restricted CTLs directed against the identified peptides (peptides are represented as first 3 residues of the sequence) were generated using peripheral blood from healthy donors. PBMCs containing the epitope specific CTLs were harvested, washed, and cultured with the peptide pulsed or HBV expressing cells overnight in an IFN-gamma ELISpot assay. Data is represented as % increase over background. (b) PBMCs containing epitope specific CTLs were harvested, washed, and cultured with uninfected or HBV expressing cells overnight in an IFN-gamma ELISpot assay. Normal liver cells served as a negative, nonspecific control.
