Indoxyl Acetate as a Substrate for Analysis of Lipase Activity

Table 1

Statistic data of concentration curves of Tween in time the range of 0–60 min.

Time (min)	Km (mmol/l)	Vmax (μkat)	R	kcat (s^−1)	LOD (mmol/l)
10	16.4	20.0	0.982	19.2	2.73
20	16.7	20.0	0.997	19.2	1.45
30	13.9	10.0	0.995	9.60	1.25
40	13.1	10.0	0.997	9.60	0.935
50	13.0	10.0	0.998	9.60	0.497
60	12.0	10.0	0.998	9.60	0.380

K_m—Michaelis–Menten constant, V_max—maximum velocity, R—correlation coefficient, k_cat—turnover number, LOD—limit of detection. The values belong to the volume 10 μl of lipase (200 U).