Indoxyl Acetate as a Substrate for Analysis of Lipase Activity

Table 2

Statistic data of concentration curves of IA in the time range of 0–30 min.

Time (min)	Km (mmol/l)	Vmax (nkat)	R	kcat (s^−1)	LOD (mmol/l)
10	13.6	95.1	0.998	0.183	3.67
15	12.3	84.7	0.999	0.163	3.15
20	11.2	74.0	0.999	0.142	3.00
25	10.4	65.7	0.999	0.126	2.89
30	9.85	59.1	0.999	0.114	2.78

K_m—Michaelis–Menten constant, V_max—maximum velocity, R—correlation coefficient, k_cat—turnover number, LOD—limit of detection. The values belong to the volume 5 μl of lipase (100 U).