Research Article
Systematic Analysis of the Whole-Body Tissue Distribution and Fatty Acid Compositions of Membrane Lipids in CD1 and NMRI Mice and Wistar Rats
Table 1
Quantitation of 11 lipid classes in 12 organs/tissues from CD1 mice, NMRI mice, and Wistar rats.
| Animal species | CD1 mice | NMRI mice | Rat | CD1 mice | NMRI mice | Rat | CD1 mice | NMRI mice | Rat | CD1 mice | NMRI mice | Rat |
| (a) Includes the data from brain, fat, heart, and kidney | Organs | Brain | Fat | Heart | Kidney | CL | 470.0 ± 63.0 | 197.8 ± 36.5 | 168.4 ± 10.9 | 8.4 ± 3.6 | 11.5 ± 7.4 | 3.2 ± 1.2 | 252.6 ± 19.8 | 220.7 ± 17.8 | 381.9 ± 32.5 | 458.0 ± 33.6 | 257.5 ± 9.2 | 444.6 ± 35.7 | LPC | 16.1 ± 2.3 | 17.0 ± 3.4 | 35.7 ± 5.0 | 2.1 ± 1.3 | 13.5 ± 9.0 | 2.5 ± 1.4 | 93.2 ± 12.2 | 115.5 ± 23.6 | 60.6 ± 22.8 | 55.2 ± 8.5 | 66.2 ± 6.2 | 31.2 ± 1.7 | LPE | 8.0 ± 1.9 | 20.4 ± 4.3 | 8.9 ± 1.6 | 0.8 ± 0.7 | 7.9 ± 8.1 | n.d. ± n.d. | 43.2 ± 4.8 | 57.0 ± 12.9 | 44.2 ± 22.0 | 48.0 ± 5.2 | 50.0 ± 7.7 | 24.8 ± 3.1 | LPS | n.d. ± n.d. | n.d. ± n.d. | 0.1 ± 0.0 | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | PC | 4079.0 ± 218.9 | 2908.9 ± 375.9 | 4912.5 ± 442.5 | 210.7 ± 36.2 | 436.5 ± 139.1 | 426.4 ± 66.6 | 2469.4 ± 125.0 | 2368.0 ± 121.1 | 4007.6 ± 244.6 | 3322.6 ± 245.9 | 3192.4 ± 68.4 | 2966.7 ± 169.7 | PE | 5120.3 ± 267.5 | 6704.8 ± 1142.4 | 13374.3 ± 556.7 | 189.2 ± 53.6 | 433.1 ± 135.7 | 246.2 ± 44.9 | 1635.3 ± 118.7 | 1638.6 ± 114.9 | 3613.5 ± 238.6 | 3377.3 ± 156.7 | 2500.0 ± 152.1 | 5495.7 ± 333.7 | PG | 95.0 ± 4.7 | 73.6 ± 6.8 | 50.4 ± 3.8 | 0.2 ± 0.2 | 3.4 ± 2.0 | 0.2 ± 0.1 | 61.8 ± 6.9 | 44.1 ± 5.4 | 49.2 ± 5.7 | 161.7 ± 32.1 | 73.9 ± 8.6 | 30.7 ± 2.2 | PI | 294.2 ± 22.9 | 235.5 ± 28.2 | 194.0 ± 10.1 | 16.0 ± 5.6 | 19.1 ± 8.2 | 8.7 ± 3.3 | 89.9 ± 8.1 | 45.0 ± 5.6 | 35.5 ± 4.6 | 405.8 ± 47.1 | 126.8 ± 9.6 | 167.4 ± 7.8 | PS | 1244.6 ± 41.4 | 1179.6 ± 87.7 | 1831.2 ± 137.0 | 59.3 ± 12.1 | 68.0 ± 12.8 | 24.5 ± 8.1 | 78.0 ± 7.4 | 44.8 ± 9.0 | 71.0 ± 15.0 | 462.9 ± 33.9 | 333.6 ± 38.2 | 606.9 ± 21.0 | DG | 20.7 ± 5.5 | 13.0 ± 4.0 | 155.1 ± 61.7 | 133.8 ± 23.5 | 92.9 ± 10.0 | 262.6 ± 36.0 | 9.9 ± 5.4 | 3.6 ± 1.1 | 47.3 ± 8.6 | 14.6 ± 2.7 | 8.1 ± 0.9 | 42.6 ± 6.8 | TG | 3.4 ± 1.5 | 26.9 ± 23.3 | 28.0 ± 10.2 | 5073.4 ± 879.5 | 3093.1 ± 308.5 | 19115.4 ± 3094.0 | 292.7 ± 99.0 | 110.8 ± 82.7 | 490.0 ± 248.2 | 231.7 ± 92.0 | 167.4 ± 53.0 | 298.8 ± 73.4 | Acidic phospholipids | 2103.8 | 1686.6 | 2244.1 | 83.9 | 101.9 | 36.6 | 482.4 | 354.6 | 537.7 | 1488.4 | 791.7 | 1249.6 | Neutral phospholipids | 9223.4 | 9651.1 | 18331.3 | 402.7 | 891.0 | 675.1 | 4241.1 | 4179.1 | 7725.9 | 6803.1 | 5808.5 | 8518.4 | Neutral lipids | 24.1 | 39.9 | 183.1 | 5207.2 | 3186.1 | 19378.0 | 302.6 | 114.4 | 537.3 | 246.3 | 175.5 | 341.4 |
| (b) Includes the data from L-intestine, liver, lung, and muscle | Organs | L-intestine | Liver | Lung | Muscle | CL | 97.2 ± 18.0 | 77.2 ± 10.1 | 64.2 ± 9.2 | 164.1 ± 25.3 | 151.6 ± 9.8 | 282.4 ± 39.2 | 35.8 ± 3.5 | 10.4 ± 1.6 | 28.7 ± 9.6 | 79.8 ± 6.4 | 55.5 ± 6.6 | 123.7 ± 17.6 | LPC | 73.7 ± 12.0 | 113.6 ± 36.5 | 101.8 ± 20.0 | 111.5 ± 25.2 | 192.7 ± 94.5 | 102.7 ± 39.4 | 15.9 ± 3.1 | 28.2 ± 6.2 | 7.9 ± 2.0 | 53.8 ± 14.6 | 22.8 ± 6.4 | 87.9 ± 36.4 | LPE | 47.8 ± 10.2 | 103.4 ± 27.1 | 17.2 ± 7.9 | 27.8 ± 9.8 | 98.8 ± 54.9 | 11.8 ± 7.3 | 10.7 ± 0.9 | 24.1 ± 6.6 | n.d. ± n.d. | 14.8 ± 5.6 | 6.6 ± 2.3 | 21.1 ± 13.8 | LPS | n.d. ± n.d. | n.d. ± n.d. | 0.2 ± 0.0 | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | 0.1 ± 0.0 | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | PC | 1728.9 ± 196.6 | 2329.0 ± 268.0 | 1977.2 ± 157.7 | 3209.5 ± 183.6 | 3795.9 ± 902.6 | 6245.5 ± 438.0 | 517.8 ± 40.8 | 855.0 ± 78.5 | 624.0 ± 191.7 | 1871.5 ± 151.3 | 1287.8 ± 84.9 | 6463.1 ± 382.7 | PE | 1546.7 ± 177.8 | 2882.2 ± 370.4 | 2463.5 ± 333.4 | 2260.5 ± 118.6 | 1942.5 ± 449.9 | 4535.3 ± 270.0 | 1511.3 ± 55.8 | 1647.3 ± 238.2 | 2658.6 ± 687.5 | 1862.0 ± 169.6 | 1471.3 ± 135.5 | 3341.1 ± 164.8 | PG | 38.8 ± 7.1 | 29.3 ± 3.6 | 15.9 ± 2.1 | 30.9 ± 9.6 | 47.7 ± 2.4 | 20.2 ± 2.6 | 449.6 ± 48.5 | 216.2 ± 28.4 | 165.4 ± 72.8 | 23.4 ± 4.0 | 15.0 ± 2.6 | 12.3 ± 1.3 | PI | 146.5 ± 22.0 | 120.9 ± 12.4 | 74.3 ± 12.3 | 104.6 ± 25.2 | 129.5 ± 7.8 | 166.1 ± 26.3 | 157.9 ± 8.1 | 43.1 ± 7.0 | 41.9 ± 18.0 | 47.3 ± 8.1 | 35.3 ± 7.1 | 30.6 ± 4.2 | PS | 278.3 ± 29.3 | 323.9 ± 43.1 | 138.2 ± 11.6 | 203.3 ± 41.1 | 124.6 ± 23.7 | 260.7 ± 113.0 | 555.9 ± 28.3 | 171.6 ± 25.4 | 381.8 ± 174.6 | 70.9 ± 8.1 | 65.0 ± 9.5 | 54.3 ± 5.9 | DG | 29.7 ± 5.1 | 24.9 ± 7.9 | 51.6 ± 9.5 | 57.8 ± 11.2 | 57.2 ± 18.4 | 101.2 ± 20.6 | 25.3 ± 3.4 | 16.7 ± 2.2 | 71.6 ± 19.3 | 8.3 ± 2.3 | 11.6 ± 3.6 | 67.8 ± 40.6 | TG | 1084.7 ± 288.1 | 1286.6 ± 475.6 | 2423.6 ± 1000.5 | 392.4 ± 96.9 | 734.6 ± 161.0 | 602.5 ± 207.8 | 323.1 ± 54.3 | 279.5 ± 92.0 | 164.5 ± 66.7 | 1894.2 ± 258.9 | 815.7 ± 277.9 | 2110.8 ± 1380.0 | Acidic phospholipids | 560.8 | 551.4 | 292.8 | 502.9 | 453.4 | 729.3 | 1199.2 | 441.5 | 617.8 | 221.4 | 170.9 | 220.8 | Neutral phospholipids | 3397.1 | 5428.2 | 4559.7 | 5609.4 | 6030.0 | 10895.4 | 2055.8 | 2554.6 | 3290.6 | 3802.1 | 2788.5 | 9913.2 | Neutral lipids | 1114.4 | 1311.4 | 2475.2 | 450.2 | 791.8 | 703.7 | 348.4 | 296.2 | 236.1 | 1902.5 | 827.3 | 2178.6 |
| (c) Includes the data from S-intestine, skin, spleen, and stomach | Organs | S-intestine | Skin | Spleen | Stomach | CL | 91.5 ± 17.9 | 23.1 ± 9.7 | 60.9 ± 21.0 | 20.3 ± 4.0 | 13.2 ± 1.7 | 18.3 ± 3.8 | 57.7 ± 5.9 | 29.7 ± 1.7 | 35.3 ± 2.5 | 44.0 ± 21.8 | 6.0 ± 2.6 | 12.7 ± 3.4 | LPC | 47.7 ± 14.3 | 71.5 ± 38.3 | 87.5 ± 34.9 | 9.4 ± 1.6 | 13.4 ± 2.1 | 7.1 ± 0.9 | 27.7 ± 6.5 | 27.5 ± 3.9 | 24.8 ± 3.0 | 329.7 ± 150.5 | 256.6 ± 86.6 | 218.0 ± 35.2 | LPE | 51.1 ± 25.4 | 111.9 ± 30.4 | 45.5 ± 21.2 | 1.9 ± 0.9 | 3.4 ± 0.6 | 1.0 ± 0.7 | 10.6 ± 2.0 | 18.2 ± 2.6 | 3.4 ± 0.9 | 272.3 ± 105.9 | 145.1 ± 56.9 | 24.0 ± 2.8 | LPS | n.d. ± n.d. | n.d. ± n.d. | 0.1 ± 0.0 | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | n.d. ± n.d. | 225.8 ± 66.0 | 23.5 ± 6.8 | 5.5 ± 2.1 | PC | 1754.5 ± 222.9 | 937.6 ± 350.3 | 2402.0 ± 1130.7 | 413.8 ± 82.5 | 691.4 ± 45.5 | 1075.8 ± 109.5 | 2084.8 ± 58.3 | 1613.7 ± 58.8 | 1670.9 ± 121.3 | 1642.8 ± 309.9 | 812.5 ± 158.7 | 879.8 ± 45.9 | PE | 1419.6 ± 184.6 | 589.4 ± 172.2 | 1865.2 ± 388.3 | 300.5 ± 30.7 | 549.4 ± 29.0 | 630.8 ± 72.0 | 1356.9 ± 60.7 | 1238.4 ± 74.1 | 1769.8 ± 50.5 | 1122.0 ± 235.3 | 582.6 ± 118.9 | 1203.4 ± 138.8 | PG | 70.9 ± 18.1 | 25.1 ± 13.1 | 7.8 ± 3.4 | 10.5 ± 2.14 | 6.2 ± 0.8 | 2.5 ± 0.4 | 37.1 ± 2.8 | 35.9 ± 2.7 | 29.9 ± 4.1 | 22.3 ± 4.8 | 16.0 ± 4.1 | 3.6 ± 0.9 | PI | 165.9 ± 29.9 | 52.1 ± 20.1 | 27.0 ± 11.7 | 44.2 ± 5.5 | 30.0 ± 2.2 | 16.1 ± 2.7 | 83.0 ± 11.3 | 52.8 ± 6.5 | 41.4 ± 3.8 | 87.2 ± 16.0 | 55.3 ± 8.9 | 14.9 ± 4.9 | PS | 231.0 ± 56.5 | 74.6 ± 15.6 | 106.0 ± 31.1 | 98.9 ± 12. | 89.1 ± 6.8 | 17.3 ± 3.7 | 298.6 ± 25.2 | 195.1 ± 25.8 | 246.1 ± 40.9 | 84.9 ± 22.3 | 47.4 ± 10.8 | 37.0 ± 9.0 | DG | 30.8 ± 6.5 | 13.6 ± 3.4 | 235.3 ± 87.3 | 12.9 ± 3.2 | 17.5 ± 2.2 | 60.7 ± 17.3 | 5.7 ± 1.5 | 4.0 ± 0.6 | 9.7 ± 0.9 | 25.3 ± 6.7 | 11.6 ± 3.3 | 27.7 ± 4.9 | TG | 616.1 ± 409.5 | 343.9 ± 233.2 | 964.7 ± 544.7 | 1316.8 ± 230.5 | 1270.5 ± 192.6 | 4080.5 ± 1091.7 | 127.7 ± 76.1 | 37.8 ± 20.0 | 133.1 ± 101.7 | 589.4 ± 288.4 | 328.2 ± 183.3 | 1365.5 ± 395.1 | Acidic phospholipids | 559.4 | 174.9 | 201.7 | 173.9 | 138.6 | 54.3 | 476.4 | 313.5 | 352.7 | 464.2 | 148.1 | 73.7 | Neutral phospholipids | 3272.9 | 1710.4 | 4400.2 | 725.6 | 1257.6 | 1714.7 | 3480.1 | 2897.8 | 3468.9 | 3366.8 | 1796.9 | 2325.3 | Neutral lipids | 647.0 | 357.5 | 1199.9 | 1329.8 | 1288.0 | 4141.3 | 133.4 | 41.8 | 142.7 | 614.7 | 339.7 | 1393.3 |
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The unit of all the measurements is ng/mg organ/tissue. Nine replicates were used for measurement.
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