Research Article
Determination of Crizotinib in Mouse Tissues by LC-MS/MS and Its Application to a Tissue Distribution Study
Table 1
The differences between the current method and some previously developed methods.
| Sample | Linear range (ng mL) | Retention time (min) | Column | Sample preparation method | Mobile phase | Reference |
| Mouse tissue | 20–8000 | 1.31 | Acquity UPLC HSS T3 column (1.8 μm, 100 mm × 2.1 mm i.d.) | Protein precipitation | Methanol (solvent A) and 0.3% formic acid water solution (solvent B) | Current method | Human plasma | 50–5000 | 4.40 | Gemini C18 column (5.0 μm, 50 × 2.0 mm i.d.) | Protein precipitation. | 10 mM ammonium bicarbonate in water and 10 mM ammonium bicarbonate in methanol-water (1 : 9, v/v) | [11] | Human plasma | 4–800 | 1.17 | CORTECS® C18 UPLC column (dp = 1.6 μm, 2.1 × 50 mm) | Solid-phase extraction | 0.01% acetic acid buffer in water (solvent A) and acetonitrile added with 10% A | [12] | Human plasma | 5–1000 | 3.80 | Accucore® C18 column (2.1 × 50 mm, 2.6 μm) | Protein precipitation | 0.1% (v/v) formic acid and acetonitrile containing 0.1% (v/v) formic acid | [13] | Human plasma | 0.1–1000 | 1.22 | ACQUITY UPLC BEH C18 column (50 × 2.1 mm, i.d., 1.7 μm) | Protein precipitation and dried under nitrogen gas | 0.1% formic acid aqueous and acetonitrile/methanol (v : v, 1 : 1) | [15] | Rat plasma | 1–2000 | 1.65 | Agilent zorbax XDB C18 column (2.1 × 50 mm, 3.5 μm) | Protein precipitation | 0.1% formic acid and methanol containing 0.1% formic acid | [16] | Mouse plasma | 10–10000 | 1.2 | Acquity UPLC® BEH C18 column (30 mm × 2.1 mm, dp = 1.7 μm) | Protein precipitation | 0.1% (v/v) ammonium hydroxide and methanol | [17] |
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