Research Article
Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus
| | Basic system | N | A | E | F |
| | Composition | Volume (μL) | Volume (μL) | Volume (μL) | Volume (μL) | | DNA polymerase | 0.5 | 25 | 0.75 | 2 | | RT enzyme mix | 2.5 | 25 | 0.4 | 2 | | RNase inhibitor | 2.5 | 25 | 0.5 | 10 | | Mg2+(25 mM) | 3 | 25 | 16 | 10 | | Reaction buffer | 9 | 25 | 16 | 10 | | Upstream primer (10 μM) | 1 | 1 | 1 | 1 | | Downstream primer (10 μM) | 1 | 1 | 1 | 1 | | Probe (10 μM) | 0.5 | 0.5 | 0.5 | 0.5 | | Sample | 5 | 5 | 5 | 5 | | Enzyme-free sterile water | Fill in up to 50 μL | Fill in up to 50 μL | Fill in up to 50 μL | Fill in up to 50 μL |
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