Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus

Table 3

Configuration of different amplification procedures.

Reaction phase	Temperature	Duration
		Amplification procedure 1 (conventional amplification procedure)		Amplification procedure 2 (rapid amplification procedure)
Degradation U-templates	25°C	10 min		2 min
Reverse transcription	50°C	15 min		3 min
Pre-denaturation	95°C	5 min		30 s
Denaturation	95°C	15 s	40 cycles	3 s	40 cycles
Annealing and extension	60°C	30 s		5 s
	Total duration	∼1 h 30 min		∼30 min