Research Article
Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus
Table 3
Configuration of different amplification procedures.
| Reaction phase | Temperature | Duration | Amplification procedure 1 (conventional amplification procedure) | Amplification procedure 2 (rapid amplification procedure) |
| Degradation U-templates | 25°C | 10 min | 2 min |
| Reverse transcription | 50°C | 15 min | 3 min |
| Pre-denaturation | 95°C | 5 min | 30 s |
| Denaturation | 95°C | 15 s | 40 cycles | 3 s | 40 cycles | Annealing and extension | 60°C | 30 s | 5 s |
| | Total duration | ∼1 h 30 min | ∼30 min |
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