Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus

Table 4

Ct values under different concentrations of primers and probes.

		Primer concentration (μM)
		0.05	0.1	0.15	0.2	0.25	0.3	0.35	0.4
SARS-CoV-2 NP probe concentration (μM)	0.05	33.24	32.97	33.34	33.22	33.08	33.20	33.04	33.18
	0.10	32.29	32.26	32.33	32.28	32.39	32.20	32.20	32.22
	0.15	32.01	31.86	32.15	31.98	32.17	31.94	32.06	31.97
	0.20	32.50	32.09	32.00	32.02	31.98	32.20	31.75	32.11
SARS-CoV-2 ORF probe concentration (μM)	0.05	34.14	34.04	34.92	36.63	35.54	39.21	N/A	N/A
	0.10	34.20	33.21	33.84	34.98	35.39	37.09	39.82	N/A
	0.15	33.19	32.24	33.86	33.73	34.09	36.70	39.31	N/A
	0.20	33.72	32.62	35.90	35.14	34.31	35.76	39.69	N/A
FluA M probe concentration (μM)	0.05	31.01	30.69	30.62	30.71	30.61	30.76	30.81	31.07
	0.10	30.55	30.27	30.02	30.18	29.98	29.73	29.93	29.98
	0.15	30.25	30.02	29.67	29.71	29.45	29.57	29.60	29.75
	0.20	30.54	29.91	29.62	29.69	29.61	29.11	29.40	29.63
FluB NS probe concentration (μM)	0.05	33.68	33.29	32.66	33.20	32.33	32.88	32.47	32.60
	0.10	33.07	32.77	32.54	32.72	32.67	32.21	32.25	32.76
	0.15	32.97	33.38	32.46	32.53	32.01	32.53	32.33	32.60
	0.20	34.03	32.55	32.63	32.68	32.62	32.98	33.02	32.87