Hyaluronidase in Clinical Isolates of Propionibacterium acnes

Figure 1

(a) Standard curve used to measure hyaluronic acid concentration. Optical density of hyaluronic acid at 540 nanometers wavelength (OD₅₄₀). (b) Standard curve used to measure hyaluronidase concentration. Hyaluronic acid will bind with albumin and form a white precipitate when acidified. When hyaluronidase is present, it digests hyaluronic acid, and the hyaluronic acid cannot bind with albumin and precipitate. Decreased precipitation is measured as increased light transmission and, therefore, a lower OD₅₄₀ reading.

(a)

(b)