Inhibition of Quorum Sensing-Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa by Culture Extract from Novel Bacterial Species of Paenibacillus Using a Rat Model of Chronic Lung Infection

Figure 4

Microscopic examination of P. aeruginosa biofilms and their inflammation in lung tissues under confocal laser scanning microscope (CLSM). (a) Negative control group showing no biofilms with normal histology. (b) Positive control group showing intense biofilms filling the alveolar spaces with severe inflammation (high magnification 100x). (c) Comparative control group showing inhibited (disrupted) biofilms with mild inflammation. (d) Extract treatment group showing clearance of biofilms with mild inflammation. Arrows indicate the components of biofilms and lung alveoli. Tissue sections stained with propidium iodide (PI) and concanavalin A (Con A).

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