Augmented β-Cell Function and Mass in Glucocorticoid-Treated Rodents Are Associated with Increased Islet Ir-β/AKT/mTOR and Decreased AMPK/ACC and AS160 Signaling

Figure 6

DEX treatment modulates proteins related to vesicle trafficking, protein synthesis, cell growth, and insulin secretion in pancreatic islets from both mice and rats. (a) Representative immunoblotting of phosphorylated and (b) total AS160 content. (c) Phosphorylated and (d) total mTOR content. (e) Phosphorylated substrates of PKC (range from 70 to 110 kDa) and (f) total PKC content. (g) CX36 expression and (h) mRNA expression of INS gene and PDX1 in islets from DEX-mice and rats. Values are mean ± S.E.M.; rodents per group. Significantly different compared to CTL. Unpaired Student’s -test, .