Conservation/Mutation in the Splice Sites of Cytokine Receptor Genes of Mouse and Human

Table 5

Columns 1–4: the nucleotide composition according to the position in the terminal hexanucleotides in Mouse and Human. Columns 1 and 5–7: analysis of the conservation of individual nucleotides in the terminal hexanucleotides of the introns. Expected random conservation in column 5 and actual conservation in column 6. Percentages are over the total of 216 couples of hexanucleotides.

1	2	3	4	5	6	7
Sequence	% in Mouse	% in Human		Random conservation (%)	Actual conservation (%)
AxxxAG	5.1	5.6		0.29	2.31	𝑃<0.01
CxxxAG	35.2	33.8		11.9	23.61	𝑃<0.01
GxxxAG	8.3	4.6	𝑃=0.01	0.38	3.70	𝑃<0.01
TxxxAG	51.4	56.0		28.78	42.59	𝑃<0.01
Total				41.35	72.21	𝑃<0.01
xAxxAG	7.9	9.3		0.73	5.09	𝑃<0.01
xCxxAG	25.9	22.2		5.75	13.89	𝑃<0.01
xGxxAG	8.3	8.3		0.69	5.09	𝑃<0.01
xTxxAG	57.9	60.2		34.86	48.61	𝑃<0.01
Total				42.03	72.68	𝑃<0.01
xxAxAG	27.3	26.9		7.34	17.13	𝑃<0.01
xxCxAG	27.3	29.1		7.94	16.67	𝑃<0.01
xxGxAG	19.9	21.3		4.24	9.26	𝑃<0.01
xxTxAG	25.5	22.7		5.79	16.67	𝑃<0.01
Total				25.31	59.73	𝑃<0.01
xxxAAG	7.4	7.0		0.52	6.02	𝑃<0.01
xxxCAG	63.0	59.7		37.6	50.46	𝑃<0.01
xxxGAG	0.0	0.0		0.00	0.00
xxxTAG	29.6	33.3		9.86	20.83	𝑃<0.01
Total				47.98	77.31	𝑃<0.01