In Vitro Evaluation of Antimicrobial Efficacy of Extracts Obtained from Raw and Fermented Wild Macrofungus, Lenzites quercina
Table 3
Zones of inhibition (mm) of raw and fermented extracts against indicator organisms at 50 mg/mL.
Tested isolates
RPP
REA
RET
FPP
FEA
FET
CPF
VAN/FLU
S. aureus (ATCC 29213)
0.0
0.0
9.0 ± 0.0
0.0
0.0
10.0 ± 0.0
15.0 ± 0.0
12.0 ± 0.0
E. coli (ATCC 35218)
12.0 ± 0.0
10.3 ± 0.5
12.7 ± 0.5
0.0
0.0
0.0
8.0 ± 0.0
10.0 ± 0.0
P. aeruginosa (ATCC 27853)
0.0
11.0 ± 0.0
13.0 ± 0.0
0.0
0.0
12.0 ± 0.0
0.0
17.0 ± 0.0
S. flexneri (ATCC 12022)
0.0
0.0
0.0
0.0
0.0
0.0
0.0
20.0 ± 0.0
S. enterica (ATCC 33458)
6.3 ± 0.0
10.0 ± 0.0
16.0 ± 0.0
10.7 ± 0.3
11.0 ± 0.0
14.7 ± 0.3
10 ± 0.0
18.3 ± 0.2
B. subtilis (ATCC 6633)
0.0
0.0
6.0 ± 0.0
0.0
14.0 ± 0.0
8.0 ± 0.0
0.0
14.0 ± 0.0
E. aerogenes (ATCC 13048)
16.2 ± 0.3
0.0
8.7 ± 0.6
10.3 ± 0.5
0.0
0.0
8.3 ± 0.6
12.0 ± 0.0
S. flexneri
12.0 ± 0.0
0.0
0.0
0.0
7.0 ± 0.0
12.0 ± 0.0
9.3 ± 0.5
12.0 ± 0.0
S. typhi
0.0
12.0 ± 0.0
8.0 ± 0.0
0.0
12.0 ± 0.0
0.0
0.0
9.0 ± 0.0
P. aeruginosa
0.0
0.0
9.0 ± 0.0
0.0
0.0
7.3 ± 0.2
0.0
10 ± 0.0
B. cereus
8.0 ± 0.0
0.0
11.0 ± 0.0
14.0 ± 0.0
0.0
9.0 ± 0.0
0.0
13.3 ± 0.3
E. faecalis
0.0
0.0
8.0 ± 0.0
0.0
10.0 ± 0.0
13.0 ± 0.0
9.3 ± 0.2
11.3 ± 0.5
E. coli
10.7 ± 0.5
0.0
0.0
14.3 ± 0.6
0.0
12.0 ± 0.0
13.0 ± 0.0
0.0
S. aureus
8.7 ± 0.5
0.0
10.7 ± 0.3
5.0 ± 0.0
11.7 ± 0.6
0.0
0.0
8.3 ± 0.0
MRSA-1
0.0
0.0
14.0 ± 0.0
0.0
0.0
11.3 ± 0.2
10.0 ± 0.4
10 ± 0.0
MRSA-2
0.0
0.0
0.0
0.0
0.0
0.0
0.0
6.0 ± 0.0
MRSA-3
0.0
0.0
0.0
0.0
0.0
0.0
0.0
11.3 ± 0.2
MRSA-4
0.0
4.0 ± 0.0
8.0 ± 0.2
0.0
0.0
0.0
0.0
6.5 ± 0.02
MRSA-5
0.0
6.0 ± 0.0
18.0 ± 0.0
0.0
6.0 ± 0.0
13.0 ± 0.0
7.0 ± 0.0
8.0 ± 0.01
MRSA-6
0.0
0.0
10.1 ± 0.0
0.0
0.0
0.0
0.0
6.0 ± 0.0
C. albicans
0.0
0.0
11.3 ± 0.0
0.0
0.0
18.0 ± 0.0
NT
20.0 ± 0.0
A. niger
8.0 ± 0.0
0.0
17.2 ± 0.1
0.0
0.0
0.0
NT
18.0 ± 0.0
A. flavus
0.0
0.0
0.0
0.0
0.0
13.0 ± 0.0
NT
8.2 ± 0.11
Values are mean ± sd of replicates (). RPP: raw Lenzites quercina extracted with pet-ether, REA: raw Lenzites quercina extracted with ethyl acetate, RET: raw Lenzites quercina extracted with ethanol, FPP: fermented Lenzites quercina extracted with pet-ether, FEA: fermented Lenzites quercina extracted with ethyl acetate, FET: fermented Lenzites quercina extracted with ethanol, CPF: ciprofloxacin (10 μg), VAN: vancomycin (30 μg), and NT: not tested; fungi are tested against FLU, fluconazole (20 mg), and 0.0, no zone of inhibition.
