Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples

Table 1

Cq (and standard deviation) obtained for different copy number of templates to evaluate the limit of detection (LoD).

Gene target	Copies per reaction
	1		5		10		25
	E	N	E	N	E	N	E	N
Replicas	18	18	18	18	18	18	18	18
Positive	7	12	11	18	18	18	18	18
Cq (mean)	40.64	39.79	39.35	36.83	36.63	35.54	34.60	33.25
SD	0.44	1.10	0.81	0.57	0.37	0.45	0.53	0.63