Miniaturized Digestion and Extraction of Surface Proteins from Candida albicans following Treatment with Histatin 5 for Mass Spectrometry Analysis

Table 2

Proteins identified from a miniaturized assay of HTN5 treatment of C. albicans with increased MS signals. Average MS signals and their standard deviations were calculated based on six replicates. Proteins indicated with an asterisk () have been determined using their associated protein families. Lower case letters in amino acid sequences indicated modified residues. Blank spaces indicated proteins unidentified by the databases.

Biological function	Observed precursor	Theoretical precursor	Ion counts		values	Amino acid sequence	Protein name	Charges
			Before	After
Surface-associated	1297.59	1298.54	18000 ± 8800	51000 ± 5400	0.00042	K.KPsTEDtFSK.Y	Peroxisomal protein family; (A0A0A6JC81)	1	1.82
Mitochondrial-associated	1297.59	1297.49	18000 ± 8800	51000 ± 5400	0.00042	R.NsIttEsVK.A	Aldehyde hydrogenase family; (A0A0A3BNV6)	1	2.03
DNA binding	575.44	576.45	9700 ± 1000	28000 ± 6200	0.00055	K.SLLSR.I	Telomerase reverse transcriptase family; (C4YDL3)	1	1.54
	1297.59	1297.75	18000 ± 8800	51000 ± 5400	0.00042	K.NVIPAtItK.V	Spt6; (D3IZV2)	1	1.54
		1298.67				K.EFTIGPFKcIK.W	Transcriptional regulatory protein family; (A0A0A3CXJ5)		1.56
Miscellaneous	575.44	576.31	9700 ± 1100	28000 ± 6200	0.00055	R.SLNSR.I	ATPase family; (A0A0A4CD62)	1	3.15
	1901.71	1903.74	7800 ± 3900	13000 ± 2300	0.025	K.NKATssSSStsRDTR.W	Leucine rich repeats; (C4YMX3)	2	3.19
Uncharacterized	1297.59	1297.69	18000 ± 8800	51000 ± 5400	0.00042	R.NNTtVSKGRIK.V	(A0A0A6M207)	1	1.51
		1297.70				K.KHQtHILNVK.S	(A0A0A6HZ28)		1.60