International Journal of Surgical Oncology / 2022 / Article / Tab 2 / Research Article
High CD44 Immunoexpression Correlates with Poor Overall Survival: Assessing the Role of Cancer Stem Cell Markers in Oral Squamous Cell Carcinoma Patients from the High-Risk Population of Pakistan Table 2 Immunoreactivity scoring criteria for all antibodies.
Antibody No. of positive cells Type of staining Scoring For statistical analysis References CD133 0 — Negative (0) Negative [35 ] <30% — Weak (+/1) Positive 30–60% — Moderate (++/2) Positive >60% — Strong (+++/3) Positive CD44 ≤10% cells Weakly stained Negative (0) Negative [36 ] 11–30% cells Weakly stained 1 (low) Low >30% weakly or <30% moderately stained 2 (low) Low 30%–60% Moderately stained 3 (high) High >60% Moderately or strongly stained 4 (high) High L1CAM 0% = 0 None = 0 Intensity × % of positive cells = score [37 ] <10% = 1 Weak = 1 0–2 = negative Negative 10–50% = 2 Moderate = 2 3–4 = weakly positive Positive 51–80% = 3 Strong = 3 6–8 = moderately positive Positive >80% = 4 9–12 = strong Positive SOX2 <10% — Negative (0) Negative [38 ] 10–50% — Weak (+/1) Positive 50–90% — Moderate (++/2) Positive >90% — Strong (+++/3) Positive