The Use of a Green Fluorescent Protein Porcine Model to Evaluate Host Tissue Integration into Extracellular Matrix Derived Bionanocomposite Scaffolds

Table 1

Qualitative and semiquantitative scoring criteria. Quantitative score is per 400x field.

Criteria	0	1	2	3
Cellular infiltration	0–50 cells	51 and 100 cells	101–150 cells	>150 cells
Multinucleated giant cells (MNGC)	0 MNGC	1-2 MNGC	3-4 MNGC	>5 MNGC
Vascularity	Either 0 or 1 blood vessel	2–5 blood vessels	6–10 blood vessels	>10 blood vessels
Connective tissue organization	Original scaffold is intact	Original scaffold disrupted; poorly organized new host ECM present	Mixture of highly organized and poorly organized connective tissue	Highly organized connective tissue present
Fibrous encapsulation	No fibrous encapsulation	Less than 50% of the periphery has fibrous encapsulation	50%–90% of the periphery has fibrous encapsulation	Complete fibrous encapsulation
Scaffold degradation	Scaffold is intact	Scaffold fibers are disrupted and present in groups or large bundles	Scaffold fibers are disrupted and present in single fibers or small bundles	No scaffold fibers remain