Research Article
Expression Pattern of Myogenic Regulatory Transcription Factor mRNAs in the Embryo and Adult Labeo rohita (Hamilton, 1822)
Table 2
Primers used for qRT-PCR of Labeo MyoD, myf-5, myogenin, MEF2A, and GAPDH.
| Gene | Primer sequence | Annealing temperature | Source sequence for primer designing (accession numbers are given) | Position of primers in source sequence |
| MyoD | Forward | 5′-TCCAAGCGCTGCTAAGAAGT-3′ | 59.0°C | Labeo rohita partial mRNA for MyoD (GenBank accession: KC344537.1) | 132–151 | Reverse | 5′-CATCATGCCATCAGAGCAGT-3′ | | 241–222 | myf-5 | Forward | 5′-GCAGGCTGAAGAAGGTGAAC-3′ | 59.0°C | Labeo rohita partial mRNA for myf-5 (GenBank accession: KC344536.1) | 96–115 | Reverse | 5′-GGCTTCCTCAGGATCTCAAC-3′ | | 195–176 | Myogenin | Forward | 5′-GGCTTCGACCAAACAGGATA-3′ | 59.0°C | Cyprinus carpio mRNA for myogenin (GenBank accession: AB012881.1) | 232–251 | Reverse | 5′-GCTCCTGGTGAGGAGACAAG-3′ | | 376–357 | MEF2A | Forward | 5′-TGACTGTGAGATTGCCCTGA-3′ | 59.0°C | Labeo rohita partial mRNA for MEF2A (GenBank accession: KC344535.1) | 94–113 | Reverse | 5′-CGTGGGGTTCGTTGTATTCT-3′ | | 206–187 | GAPDH | Forward | 5′-AGGGGCTCAGTATGTTGTGG-3′ | 59.0°C | Cyprinus carpio partial mRNA for GAPDH (GenBank accession: AJ870982.1) | 257–276 | Reverse | 5′-CTCTCTTGGCACCACCCTTA-3′ | | 342–323 |
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