Expression Pattern of Myogenic Regulatory Transcription Factor mRNAs in the Embryo and Adult Labeo rohita (Hamilton, 1822)

Table 2

Primers used for qRT-PCR of Labeo MyoD, myf-5, myogenin, MEF2A, and GAPDH.

Gene	Primer sequence		Annealing temperature	Source sequence for primer designing; (accession numbers are given)	Position of primers in source sequence
MyoD	Forward	5′-TCCAAGCGCTGCTAAGAAGT-3′	59.0°C	Labeo rohita partial mRNA for MyoD; (GenBank accession: KC344537.1)	132–151
	Reverse	5′-CATCATGCCATCAGAGCAGT-3′			241–222
myf-5	Forward	5′-GCAGGCTGAAGAAGGTGAAC-3′	59.0°C	Labeo rohita partial mRNA for myf-5; (GenBank accession: KC344536.1)	96–115
	Reverse	5′-GGCTTCCTCAGGATCTCAAC-3′			195–176
Myogenin	Forward	5′-GGCTTCGACCAAACAGGATA-3′	59.0°C	Cyprinus  carpio mRNA for myogenin; (GenBank accession: AB012881.1)	232–251
	Reverse	5′-GCTCCTGGTGAGGAGACAAG-3′			376–357
MEF2A	Forward	5′-TGACTGTGAGATTGCCCTGA-3′	59.0°C	Labeo rohita partial mRNA for MEF2A; (GenBank accession: KC344535.1)	94–113
	Reverse	5′-CGTGGGGTTCGTTGTATTCT-3′			206–187
GAPDH	Forward	5′-AGGGGCTCAGTATGTTGTGG-3′	59.0°C	Cyprinus  carpio partial mRNA for GAPDH; (GenBank accession: AJ870982.1)	257–276
	Reverse	5′-CTCTCTTGGCACCACCCTTA-3′			342–323