Ulcerative Colitis and Its Association with Salmonella Species

Table 1

Primers used for amplification of S. Typhi and S. Paratyphi A serotypes in clinical specimens.

Primer sequence	Target gene	Salmonella serotype	Amplicon size (bp)	PCR condition (number of cycles)	References
ST1 5′-ACTGCTAAAACCACTACT-3′; ST2 5′-TTAACGCAGTAAAGAGAG-3′	fliC	Typhi	495 bp	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35)	Song et al. [17]
ST3 5′-AGATGGTACTGGCGTTGCTC-3′; ST4 5′-TGGAGACTTCGGTCGCGTAG-3′			364 bp	94°C, 1 min; 63°C, 1 min; 72°C, 1 min (35)	Frankel [18]
staA F1: 5′-TGGTTACATGACCGGTAGTC-3′; staA R1: 5′-TAGCTGCCGCAATGGTTATG-3′	staA	Typhi	537 bp	94°C, 1 min; 57°C, 1 min; 72°C, 1 min (35)	Pratap et al. [19]
staA F2: 5′-CATCGGCACGAACGTAAGAC-3′; staA R2: 5′-TCAAGCGACTGATGGTGACG-3′			377 bp	94°C, 1 min; 65°C, 1 min; 72°C, 1 min (35)
stkG F1: 5′-CGTTTACTGAGGTCACAGGCATC-3′; stkG R1: 5′-CACATTGTTCTCGGAGACCCCA-3′	stkG	Paratyphi A	427 bp	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35)	Pratap et al. [20]
stkG F2: 5′-CAATGGCTTCTGGCGAACTGTC-3′; stkG R2: 5′-TGGAGAAAGATCAGACCACCGAG-3′			229 bp	94°C, 1 min; 61°C, 1 min; 72°C, 1 min (35)