Simultaneous Preconcentration of Fast Green FCF and Rhodamine B Using Deep Eutectic Solvent and Determination via High-Performance Thin Layer Chromatography

Table 4

Analysis of variance results for simultaneous preconcentration of fast green FCF and rhodamine B.

Source	Sum of squares	D.F^1	Mean square	F value	p value
Fast green FCF
Model	8.629E + 006	14	6.164E + 005	956.71	<0.0001	Significant
A (pH)	8719.19	1	8719.19	13.53	0.0022
B (volume of deep eutectic solvent)	1.78E + 05	1	1.78E + 05	276.54	<0.0001
C (concentration of salt)	6.37E + 05	1	6.37E + 05	988.59	<0.0001
D (stirrer time)	21090.05	1	21090.05	32.73	<0.0001
AB	96744.33	1	96744.33	150.16	<0.0001
AC	196.35	1	196.35	0.3	0.589
AD	20653.28	1	20653.28	32.06	<0.0001
BC	5.70E + 05	1	5.70E + 05	885.37	<0.0001
BD	21035.88	1	21035.88	32.65	<0.0001
CD	899.25	1	899.25	1.4	0.2558
A^2	2.77E + 06	1	2.77E + 06	4303.69	<0.0001
B^2	1.68E + 06	1	1.68E + 06	2601.86	<0.0001
C^2	6.36E + 05	1	6.36E + 05	986.39	<0.0001
D^2	4.41E + 06	1	4.41E + 06	6844.58	<0.0001
Residual	9664.09	15	644.27
Lack of fit	8347.39	10	834.74	3.17	0.1074	Not significant
Pure error	1316.7	5	263.34
Total	8.64E + 06	29
Rhodamine B
Model	8.455E + 006	14	6.039E + 005	455.35	<0.0001	Significant
A (pH)	1.107E + 005	1	1.107E + 005	83.47	<0.0001
B (volume of deep eutectic solvent)	1.10E + 05	1	1.10E + 05	82.8	<0.0001
C (concentration of salt)	9.64E + 05	1	9.64E + 05	727.06	<0.0001
D (stirrer time)	68.01	1	68.01	0.051	0.8239
AB	1.70E + 05	1	1.70E + 05	128.07	<0.0001
AC	98863.08	1	98863.08	74.54	<0.0001
AD	1434.52	1	1434.52	1.08	0.3148
BC	3.77E + 05	1	3.77E + 05	284.38	<0.0001
BD	5.29E + 05	1	5.29E + 05	398.98	<0.0001
CD	1.77E + 05	1	1.77E + 05	133.09	<0.0001
A^2	2.06E + 05	1	2.06E + 05	155.14	<0.0001
B^2	2.79E + 06	1	2.79E + 06	2103.05	<0.0001
C^2	1.16E + 05	1	1.16E + 05	87.35	<0.0001
D^2	3.25E + 06	1	3.25E + 06	2447.51	<0.0001
Residual	19893.5	15	1326.23
Lack of fit	11130.83	10	1113.08	0.64	0.7472	Not significant
Pure error	8762.67	5	1752.53
Total	8.47E + 06	29

¹Degree of freedom.