A Combination of Human Embryonic Stem Cell-Derived Pancreatic Endoderm Transplant with LDHA-Repressing miRNA Can Attenuate High-Fat Diet Induced Type II Diabetes in Mice

Figure 2

Characterization of hESC-derived PE and miR function. (a) miR-590-3p transfected H1 ESCs (miR-H1-ESC) were differentiated to pancreatic endoderm (PE) (see Section 2). Cells of undifferentiated miR-H1-ESC and differentiated PE (miR-PE) were subject to immunofluorescence analysis and stained for DAPI, OCT4, and PDX1. (b) miR-PE stained for DAPI, SOX9, and FOXA2. (c) Relative gene expression of OCT4, PDX1, FOXA2, and SOX9 from miR-hESCs and miR-PE. All values are given as mean; error bar represents standard error. , compared with undifferentiated H1 hESC. (d) Real-time qPCR results of LDHA expression in H1-ESC with and without miR-590-3p transfection and doxycycline induction. All values are given as mean; error bar represents standard error. , compared with nontransduced H1 hESC. (e) Western blot analysis of LDHA in miR-PE cells with/without doxycycline induction.

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