Research Article
The Autoantigenic Proinsulin B-Chain Peptide B11-23 Synergises with the 70 kDa Heat Shock Protein DnaK in Macrophage Stimulation
Table 2
Release of IL-6, TNFα, and IL-1β from MonoMac 6 cells exposed to proinsulin-derived 13mer peptides alone or in combination with DnaK.
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MonoMac 6 cells were incubated in the absence (medium) or in the presence of LPS (10 ng/ml) or DnaK (1 μg/ml) in the absence or presence of proinsulin peptides (10 μg/ml). Concentrations of TNFα were determined after 6 h of incubation and concentrations of IL-6 and IL-1β were determined after 24 h of incubation in the culture supernatants. Results from peptide-DnaK combinations were corrected by cytokine levels induced by stimulation with DnaK alone. Data are given as means ± SD from three experiments performed in triplicates. The multiplex system used here for cytokine measurements yielded lower results than ELISA (Figure 2), but the relative differences between LPS, peptide B11-23, and peptide B11-23 + DnaK were not affected. for the difference to medium. for the differences between peptide and peptide + DnaK. for the differences between peptide and peptide + DnaK. |