Quercetin Inhibits Pyroptosis in Diabetic Cardiomyopathy through the Nrf2 Pathway

Figure 3

Quercetin promotes the nuclear translocation of Nrf2. (a) Expression levels of Nrf2 in the nucleus detected by WB. (b) Expression levels of HO-1 and GCLC detected by WB. (c) Immunofluorescence detection of the expression level of HO-1 in cells ( magnification). (d) Viability of cells in each group detected by CCK-8 assay. H3: histone H3, used as a reference nuclear protein for normalization. CON-G: glucose concentration of 5.5 mmol/L; H-G: glucose concentration of 30 mmol/L; Que-G: glucose concentration of 30 mmol/L+quercetin; WB: western blotting. Data are expressed as the . , H-G vs. Con-G and Que-G. ^#, Que-G vs. Con-G. ^{^}, Que-G vs. Con-G and H-G.

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