Quercetin Inhibits Pyroptosis in Diabetic Cardiomyopathy through the Nrf2 Pathway

Figure 4

ML385 inhibits the nuclear translocation of Nrf2. (a) mRNA expression levels of HO-1 and GCLC detected by PCR. (b) Expression of Nrf2 in the nucleus detected by WB. (c) Expression levels of pyroptosis pathway-related proteins detected by WB. (d) Expression of NLRP3 and HO-1 in cells detected by immunofluorescence (). (e) A TUNEL fluorescent probe was used to detect the incidence of apoptosis in each group (). H-G: glucose concentration of 30 mmol/L; Que-G: glucose concentration of 30 mmol/L+quercetin; IH+Que-G: glucose concentration of 30 mmol/L+quercetin+ML385. Data are expressed as the . ^{^}, Con-G vs. ML385. , Que-G vs. H-G and IH+Que-G. ^#, IH+Que-G vs. H-G.

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