Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Figure 3

Effects of microbe-derived products and whole yeasts on the release of S100A8/A9 and S100A12. (a) C. albicans (10⁸ cells/mL) enhances the secretion of S100A8/A9 and S100A12, whereas zymosan (1.5 mg/mL) induces only the release of S100A8/A9. (b) Pam3CSK4 (TLR2 ligand, 10 μg/mL) potentiates the release of S100A8/A9 but not S100A12. Neutrophils were incubated with whole microorganisms or microbe-derived product for 60 min at 37°C. The concentrations of secreted S100A8/A9 and S100A12 were determined by ELISA. Results are the mean ± SEM of at least three independent experiments performed on neutrophils from different donors. and compared with control by paired -test.

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