Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Figure 7

S100A8, S100A9, S100A12, and calprotectin move toward cytoskeleton/lipid raft fractions following neutrophils stimulation by H₂O₂ or MSU. Neutrophils were stimulated with 1.5 mg/mL MSU crystals or 250 μM H₂O₂ and supernatants harvested before sequential cells lysis as described in Section 2. Fractions were loaded onto SDS-PAGE and (a) β actin, α tubulin, and flotillin-1 or (b) S100A8/A9 or S100A12 was detected by immunoblotting. Results are from one experiment are representative of 2 others. (c) Fractions were subjected to sandwiches ELISA against S100A8, S100A9, S100A12, or S100A8/A9. Results represent the mean ± SEM of 4 donors quantified in duplicate. ; ; , MSU versus HBSS.

(a)

(b)

(c)