Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Figure 8

S100A8/A9 and S100A12 are almost absent from microvesicles. Neutrophils were incubated with MSU crystals (1.5 mg/mL) or its diluent for 60 min at 37°C. The MV were isolated after sequential centrifugation. The concentrations of (a) S100A8/A9 and (b) S100A12 were determined by ELISA in the supernatant fractions (S1, S2, S3, and S4) and the pellet fractions (P1, P2, and P3) containing the MV. Data are the mean ± SEM of three separate experiments. (c) The presence of S100A8 and S100A9 in the supernatants and MV was determined by western blot. Results are from one experiment representative of 2 others.

(a)

(b)

(c)