Preparation and Biological Activity of the Monoclonal Antibody against the Second Extracellular Loop of the Angiotensin II Type 1 Receptor

Figure 4

Biological activity of AT1-mAb was identified by isolated thoracic aorta ring technology. (a) Negative IgG isolated from healthy mouse serum; (b) Agonist of AT₁R, Ang II; (c) AT1-mAb; (d) for AT1-AA exert the same effect on the contractile force of mouse thoracic aorta; (e) blocking agent for AT₁R, valsartan; (f) valsartan + AT1-mAb; (g) blocking agent for AT₂R, PD123319; (h) PD123319 + AT1-mAb; and (i) statistical graph of isolated vascular ring. versus negative IgG. Data represent the mean ± SEM; .

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