Differential Use of Human Neutrophil Fcγ Receptors for Inducing Neutrophil Extracellular Trap Formation

Figure 10

FcγRIIa induces efficient phagocytosis. (a) Human neutrophils were mixed with fluorescence latex particles. Particles were nonopsonized (no Ab) or opsonized with monoclonal antibody (mAb) IV.3 anti-FcγRIIa or with mAb 3G8 anti-FcγRIIIb. Cells were allowed to ingest the particles for 30 min. Phagocytosis of latex beads was also evaluated in the absence (None) or the presence of 50 μM UO126 (MEK inhibitor) or 2.5 μM BAY 117082 (NF-κB inhibitor). Phagocytosis was assessed by flow cytometry, detecting the reduced number of cells with low fluorescence (M1 marker), and the appearance of cells with high fluorescence in the far right side (M2 marker) of the histogram of gated PMN. (b) Phagocytosis was quantified by flow cytometry, as the percentage of high-fluorescence cells (marker M2) in the histogram of gated PMN. Data are representative of four separate experiments.

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