Differential Use of Human Neutrophil Fcγ Receptors for Inducing Neutrophil Extracellular Trap Formation

Figure 3

NETs are decorated with neutrophil elastase. Human neutrophils (PMN) were stimulated with 20 nM phorbol 12-myristate 13-acetate (PMA), by cross-linking FcγRIIa with mAb IV.3, by cross-linking FcγRIIIb with mAb 3G8, by cross-linking β1 integrins with mAb TS2/16, or by cross-linking β1 integrins with mAb IB4. After four hours, PMN were fixed and stained for DNA (DAPI, red) or for elastase (green). Microphotographs were taken at 200x magnification and are representative of five experiments. Bar is 50 μm.