Differential Use of Human Neutrophil Fcγ Receptors for Inducing Neutrophil Extracellular Trap Formation

Figure 6

FcγR-cross-linking induces activation of ERK. Human neutrophils (PMN) were left untreated (—) or were stimulated (a) with 20 nM phorbol 12-myristate 13-acetate (PMA) or (b) by cross-linking FcγRIIa with mAb IV.3 or (c) by cross-linking FcγRIIIb with mAb 3G8. PMN were also stimulated in the presence of the MEK inhibitor UO126 (50 μM). PMN cell lysates were prepared after 30 min stimulation. Proteins were resolved by SDS-PAGE and then Western blotted for phosphorylated-ERK (p-ERK) (upper panel) or for total ERK (lower panel). Data are representative of three separate experiments.

(a)

(b)

(c)