Research Article
Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation
Table 1
List of unique peptides and MRMs for ADA isotopes/subclasses used in the immunocapture-LC/MS assay.
| Isotype/subclass | Unique peptide sequence | MRM pairs |
| Quantitation peptide | IgG1 | GPSVFPLAPSSK | 593.83→699.40 | IgG2 | GLPAPIEK | 412.75→654.38 | IgG3 | WYVDGVEVHNAK | 708.85→698.48 | IgG4 | GLPSSIEK | 415.73→660.36 | IgE | AEWEQK | 395.69→590.29 | IgM | GQPLSPEK | 428.23→670.38 | IgA1, IgA2 | YLTWASR | 448.73→620.32 |
| Confirmation peptide | IgG1, IgG3 | ALPAPIEK | 419.76→654.38 | IgG1, IgG3, IgG4 | VVSVLTVLHQDWLNGK | 603.34→1110.57 | IgE | LEVTR | 309.18→375.24 | IgM | VSVFVPPR | 450.77→615.36 | IgA1, IgA2 | VAAEDWK | 409.71→648.30 | IgA1 | TFTCCAMTAAYPESK | 688.31→765.38 |
|
|