PM2.5 Causes Increased Bacterial Invasion by Affecting HBD1 Expression in the Lung

Figure 3

Effect of PM2.5 on bacterial infestation. (a) The effect of PM2.5 on the bacterial invasiveness of PAO1-infested HBE was examined by the colony method. Different concentrations (0, 50, 100, 200 μg/mL) of PM2.5 stimulated HBE cells for 24 hr, and then the configured PAO1 bacterial solution was added to the exposure model at different infection complexes (MOI = 1, 10, 20) (n = 3). (b) The number of intracellular viable bacteria after PAO1 infection with HBE stimulated by different concentrations of PM2.5 (0/25/50/100 μg/mL) (n = 3). (c) The mice were modeled with gross lung and HE staining performance (c1–c4) in the order of control, PM2.5, PAO1, and PAO1 + PM2.5 groups (n = 6). (d) For HE staining microscopic magnification × 40x observation (d1–d4) in order of control, PM2.5, PAO1, and PAO1 + PM2.5 groups (n = 6). , , vs. control; ^&&& PAO1 + PM2.5 vs. PAO1; ^### PAO1 + PM2.5 vs. HBD1 (one-way ANOVA). (e) H&E staining of mouse lung tissue for lung injury scoring (n = 6).

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