PM2.5 Causes Increased Bacterial Invasion by Affecting HBD1 Expression in the Lung

Figure 6

Changes in bacterial invasiveness in the PM2.5+PAO1 cell model in the presence of pathway inhibitors. (a) Cell lysate dilution coating plate colony count (n = 3). (b) Detection of DEFB1 mRNA expression in lung tissue homogenates by RT–qPCR (n = 3). (c) Laser confocal microscopy photographed intracellular live bacterial infection in each group under the effect of inhibitors. Yellow arrows indicate PAO1. (c1–c5) PAO1 + PM2.5 group; U0126 + PAO1 + PM2.5 group; SB203580 + PAO1 + PM2.5 group; SP600125 + PAO1 + PM2.5 group; BAY11-7082 + PAO1 + PM2.5 group, in that order (scale bars = 20 µm, n = 3). (d) The number of viable intracellular bacteria was counted under laser confocal microscopy (n = 3). , , vs. PM2.5 + PAO1 (t test).

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