Research Article
Solubilization and Humanization of Paraoxonase-1
Table 2
Activity of hum-4E9. kcat/Km values were determined under constant 2% methanol conditions for all three substrates. None of the enzymes could be saturated with those substrates under experimental conditions. SP-CMP specific activities were determined at ~0.0125 mM SP-CMP. GF kapp hydrolysis rate constants and stereoisomeric preferences were determined using GF at 0.315 mM. ND: not determined.
|