Review Article

New Insights on Biosynthesis of Nanoparticles Using Plants Emphasizing the Use of Alfalfa (Medicago sativa L.)

Table 2

Synthesis of different nanoparticles using Medicago sativa L. and other plant extracts from callus cultures.

Nr.PlantsPlant’s part/simple sizeNanoparticleShapeNanoparticle size (nm)Precursor solutionCharacterization/extraction techniquesAdditional catalyst(s) or control variablesApplications of NPsReference

1Medicago sativa L.Callus cultures from seeds/a known massAgSpherical, disk, irregular2–50AgNO3UV-Vis, TEM, FTIR/fresh callus soaked in nonaerated silver nitrate for extractionTime, room temperature, no light exposure, Ag precursor concentration, ratio of pHHegazy et al. [65]
2Carica papaya L.Callus cultures (CC) of shoot-tips and leaf-bits/20 gAgSpherical60–80AgNO3FTIR, SEM, XRD/crushing in sterile distilled water for extractionTime, room temperature, amount of extract, Ag precursor concentrationMude et al. [66]
3Sesuvium portulacastrum L.CC of whole plant/20 gAgSpherical5–20AgNO3UV-Vis, TEM, FT-IR/centrifugation for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureAntimicrobial activityNabikhan et al. [67]
4Arachis hypogaea L.CC of leaves/20 gAuSpherical31–50HAuCl4UV-Vis, XRD, EDS, SAED/centrifugation for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureRaju et al. [68]
5Justicia jendaracia (Burm.f)CC of seedlings/25 gAgSpherical50–65AgNO3UV-Vis, SEM/crushing in sterile distilled water for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureAntibacterial activityNamasivayam et al. [69]
6Michelia champaca L.CC of fresh flowers/25 gAg, AuMultiple shapes1–200HAuCl4, AgNO3UV-Vis/for extraction
TEM, XRD, DLS, ICP-OES, FTIR/crushing in sterile distilled water for extraction
Time, ratio of extract and precursor, centrifugation parameters, temperatureAntimicrobial activityIndira-Iyer and Panda [70]
7Linum usitatissimum L.CC of whole plant/10 g of CCAgSpherical19–24
49–54
AgNO3UV-Vis, FT-IR, XRD TEM, FT-IR/crushing in sterile distilled water for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureBactericidal activityAnjum and Abbasi [38]
8Taxus yunnanensis Zucc.CC of whole plant/50 gAgSpherical6.4–27.2AgNO3UV-visible, TEM, XRD, FTIR/crushing in sterile, boiled distilled water for extractionTime, amount of extract and precursor, centrifugation parameters, temperature, pHAntibacterial activity, anticancer activityXia et al. [71]
9Couroupita guianensis Aubl.CC of leaves/10 gAgSpherical30.38–88.32AgNO3UV-visible, SEM, AFM, FTIR/crushing in sterile, boiled distilled water for extractionTime, amount of extract and precursor, centrifugation parameters, temperature, pHAntimicrobial potentialKumar et al. [72]
10Viola canescens Wall.CC of leaves, petiole/12 gZnOHexagonal0.9Zn (NO3)2UV-visible, XRD, FTIR, SEM/heating for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureAntibacterial activityKhajuria et al. [73]
11Hyptis suaveolens (L.) KuntzeCC of whole plant/20 gAgSpherical12–25AgNO3UV-visible, EDS, SEM, TEM, XRD, FTIR/heating for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureAnticancer activityBotcha and Prattipati [74]
12Cinnamomum camphora Nees.CC of plantlets/1 gAgSpherical5.47–9.48AgNO3UV-Vis, TEM, SEM-EDX, DLS, FT-IR, XRD/incubation under different types of light for extractionTime, amount of extract and precursor, centrifugation parameters, temperature, pHAntibacterial activitiesAref and Salem [64]
13Lithospermum erythrorhizon Siebold, Zucc.CC of whole plant/0.1 g of dry simpleAg, AuSpherical20–50AgNO3, HAuCl4UV-Vis, XRD, TEM, EDX/boiled water for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureAnticancer activityShkryl et al. (2021) [75]
14Alhagi maurorum Medik.CC of seeds/a known massAgSpherical16–30AgNO3UV–VIS, FTIR, XRD, TEM/stirring for extractionTime, amount of extract and precursor, temperatureAntimicrobial activitySehrawat et al. [76]
15Solanum incanum L.CC of leaves and petioles/a known massAgSpherical, crystallographic15–60AgNO3UV-Vis, FT-IR, XRD TEM, FT-IR/heating for extractionTime, amount of extract and precursor, centrifugation parameters, temperatureAntimicrobial, cytotoxic efficacyLashin et al. [77]
16Bunium persicum (Boiss.) Pimenov & KljuykovCC of seeds/20 gAgSpherical35–77AgNO3UV-visible, SEM, XRD, FTIR, EDX, TGA/heating for extractionTime, amount of extract and precursor, centrifugation parameters, temperature, pHAntibacterial activityKhan et al. [61]
17Randia aculeata LCC of leaves/a known massAgSpherical10–40AgNO3UV-VIS, DLS, TEM, FTIR/heating for extractionTime, amount of extract and precursor, centrifugation parameters, temperature, pHAntibacterial, antiproliferative activityBernabé-Antonio [78]