Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy

Figure 5

Statistical analysis of the temporal behavior of the normalized two-photon fluorescence value S_damage,TPF observed at the nucleus of HeLa cells. (a) Averaged temporal behaviors of the normalized two-photon fluorescence values of the nucleus of HeLa cells with the single-focus excitation (blue dashed line), the multifocus excitation (red solid line), and the control condition (green one-dotted chain line). Shaded area indicates standard deviation. value of Student’s t-test comparing (b) the single-focus excitation and the control condition, (c) the multifocus excitation and the control condition, and (d) the single- and the multifocus excitations.

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