Rapid Screening and Estimation of Binding Constants for Interactions of Fe³⁺ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Table 1

Percent relative standard deviation (% RSD) of mobility ratios recorded from transferrin- and apotransferrin-iron (III) interactions at different iron (III) ion concentrations.

Iron (III) concentrations (μmol/L)	Percent relative standard deviation
	Transferrin		Apotransferrin
	Rf	Ri	Rf	Ri
5	1.28	0.60	0.19	0.11
10	0.61	0.70	0.18	0.66
25	0.37	0.16	0.13	0.37
50	1.22	0.94	0.91	0.54
100	0.84	0.54	0.03	0.53
150	1.08	0.28	0.88	0.10
250	1.23	1.41	0.14	0.18

n = 6.