Estrogen Regulates MAPK-Related Genes through Genomic and Nongenomic Interactions between IGF-I Receptor Tyrosine Kinase and Estrogen Receptor-Alpha Signaling Pathways in Human Uterine Leiomyoma Cells

Figure 2

IGF-I mRNA and IGF-I peptide expression levels induced by 17𝛽-estradiol (E₂) in UtLM cells. (a) IGF-I gene expression in UtLM cells following E₂ treatment at 0 (control), 10 and 60 min, and 24 h and 48 h. (b) IGF-I protein expression in UtLM cells following E₂ exposure at 0 (control), 10 and 60 min, and 24 h and 48 h. Inset: Negative control with normal mouse IgG. Representative of mean ± SEM from three independent experiments. *𝑃≤0.05 versus control.

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