Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO_i Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway

Figure 1

Contractile activity is essential for iNOS expression. (a) NRVMs were treated with L-type calcium channel blockers (nifedipine 5–10 μM or verapamil 5–10 μM) or myosin II ATPase inhibitors (butanedione monoxime (BDM) 3.75–7.5 mM or blebbistatin 5–10 μM) for 24 h. Cell extracts (50 μg total protein per lane) were then separated by SDS-PAGE and Western blotting with antibodies specific for iNOS, eNOS, and GAPDH. (b and c) The quantitative results of 5 experiments are depicted. *𝑃<0.05 versus. untreated, control cells. (d) NO_i was measured under control conditions and in the presence of 7.5 mM BDM, 10 μM blebbistatin or 10 μM nifedipine. The quantitative results of 5 experiments are depicted. *𝑃<0.05 versus untreated, control cells. ^#𝑃<0.05 versus Nifedipine-treated cells.

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