Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and
NOi Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway
Figure 2
Contraction-induced iNOS expression is regulated by FAK and AKT. (a) NRVMs were maintained in control medium (con) or treated with PF 573228 (PF, 10 M) or LY 294002 (LY, 10 M) for 24 h. Cell extracts (50 μg total protein per lane) were then separated by SDS-PAGE and Western blotting with antibodies specific for iNOS and GAPDH. (b) The quantitative results of 5 experiments are depicted. * versus control. (c) NRVM were infected, (20 moi) with Adv-expressing shRNAs for luciferase (shLuc) or FAK (shFAK) and then maintained under control conditions for 72 h. Equal amounts of cell extracts (50 μg total protein per lane) were then separated by SDS-PAGE and Western blotting with antibodies specific for FAK, iNOS, and GAPDH.
