Genome-Wide Prediction of Vaccine Candidates for Leishmania major: An Integrated Approach

Table 1

List of predicted 25 L. major candidate antigens and 5 control antigens along with their prediction probabilities using SOLpro and TMHMM program.

Antigen number	GeneDB ID/UniProt accession number	Number of amino acids	Function	Protein solubility upon overexpression prediction probabilities	Number of TM regions
1	LmjF.03.0550	1316	Hypothetical protein, conserved	0.947197; Insoluble	5
2	LmjF.04.0130	531	Hypothetical protein, conserved in Leishmania	0.814123; Insoluble	0
3	LmjF.04.0180	504	Surface antigen-like protein	0.819598; Soluble	1
4	LmjF.04.0190	709	Surface antigen-like protein	0.931571; Insoluble	1
5	LmjF.04.0200	182	Surface antigen-like protein	0.769865; Soluble	0
6	LmjF.04.0210	277	Surface antigen-like protein	0.913911; Insoluble	2
7	LmjF.04.0910	248	Hypothetical protein, conserved	0.586919; Soluble	0
8	LmjF.06.0380	401	Hypothetical protein, conserved	0.76955; Insoluble	0
9	LmjF.09.0850	335	Ras family protein-like protein	0.612666; Insoluble	0
10	LmjF.12.0710	108	Hypothetical protein, conserved	0.556695; Insoluble	1
11	LmjF.12.1000	385	Promastigote surface antigen protein 2, PSA2	0.802331; Insoluble	2
12	LmjF.12.0870	547	Surface antigen protein 2, putative	0.696157; Soluble	2
13	LmjF.12.0740	760	Surface antigen protein, putative	0.534869; Soluble	2
14	LmjF.13.0480	522	Hypothetical protein, conserved	0.785913; Soluble	0
15	LmjF.14.0770	396	Hypothetical protein, unknown function	0.705749; Insoluble	0
16	LmjF.16.0620	1136	Hypothetical protein, unknown function	0.682847; Soluble	0
17	LmjF.17.1350	179	Hypothetical protein, conserved	0.625526; Insoluble	1
18	LmjF.22.0470	426	Hypothetical protein, conserved	0.743393; Soluble	0
19	LmjF.22.1260	1087	Hypothetical protein, conserved	0.689942; Insoluble	0
20	LmjF.23.0225	221	Hypothetical protein, conserved	0.870557; Insoluble	1
21	LmjF.24.1520	1152	Hypothetical protein, conserved	0.588925; Insoluble	0
22	LmjF.26.0340	342	Hypothetical protein, conserved	0.671966; Insoluble	1
23	LmjF.28.2565	264	Hypothetical protein, conserved	0.607781; Insoluble	0
24	LmjF.32.0510	363	Hypothetical protein, conserved	0.825034; Insoluble	2
25	LmjF.33.1890	556	Hypothetical protein, conserved	0.558399; Insoluble	1
CSP	P02893	412	Circumsporozoite protein	0.857803; Soluble	0
M1	P36347	252	Matrix protein 1	0.655264; Insoluble	0
NP	P03466	498	Nucleoprotein	0.528252; Soluble	0
Core	CAA59535.1	185	HBV core	0.816707; Insoluble	0
PSTA1	P9WG10	304	Phosphate transport system permease protein 1	0.654434; Insoluble	6